| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.084726 |
| Chromosome: | chromosome 5 |
| Location: | 1194977 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g245451 | RBL10 | Rhomboid-like protease; (1 of 11) 3.4.21.105 - Rhomboid protease | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTAGCAGTGCCTGTTGCTGAGGCTGAGGC |
| Internal bar code: | TAGAGGGCGTGGAGGCCTCTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 274 |
| LEAP-Seq percent confirming: | 99.38 |
| LEAP-Seq n confirming: | 1603 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGCCCAAACTAAACCAAA |
| Suggested primer 2: | CTCTAGGAACTGGTCTGCCG |