| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.084760 |
| Chromosome: | chromosome 5 |
| Location: | 657641 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g230971 | gene_edge/mRNA_edge/3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCAAATAGGTGCTTCACACACTATGCTG |
| Internal bar code: | ATCACGGACGTGCGTAGGTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 626 |
| LEAP-Seq percent confirming: | 98.7302 |
| LEAP-Seq n confirming: | 311 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCGCTGAGCTGAGGTTGTT |
| Suggested primer 2: | GTAGCTTCCTCCCAGAGCCT |