Insertion junction: LMJ.RY0402.084763_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.084763
Chromosome:chromosome 7
Location:3324768
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g334750 PPP30 (1 of 3) PTHR12320//PTHR12320:SF15 - PROTEIN PHOSPHATASE 2C // SUBFAMILY NOT NAMED; Phosphoprotein phosphatase 2C-related intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGAAACATCAGTACAGATTGCCATCGCGA
Internal bar code:TCGGATTAACTCTCTTCTCGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:922
LEAP-Seq percent confirming:99.3822
LEAP-Seq n confirming:1287
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:AGACAACAGACAACCCCAGG
Suggested primer 2:ACAAGACCCACGACAAGACC