| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.084784 |
| Chromosome: | chromosome 1 |
| Location: | 2761963 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g016400 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCACCGCACCGTACGAGTCGCCCACAGC |
| Internal bar code: | GTACACTGGGTCGCGGCAAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 198 |
| LEAP-Seq percent confirming: | 78.764 |
| LEAP-Seq n confirming: | 1402 |
| LEAP-Seq n nonconfirming: | 378 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGGGCTATGAGCATCAAT |
| Suggested primer 2: | GAGCTCAGAGGCTCAACAGG |