| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.084793 |
| Chromosome: | chromosome 13 |
| Location: | 3874456 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g590251 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATGGCTTGTGATCCATGCTTTCTGTTGTG |
| Internal bar code: | ACTGCGCTGTGGGTTAGGGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 839 |
| LEAP-Seq percent confirming: | 98.8833 |
| LEAP-Seq n confirming: | 5933 |
| LEAP-Seq n nonconfirming: | 67 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGATATGAGGCAGCAGCAA |
| Suggested primer 2: | CAGCGCAGATGGAAGATACA |