Insertion junction: LMJ.RY0402.084824_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.084824
Chromosome:chromosome 7
Location:5798880
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g353200 (1 of 1) IPR006073//IPR016496//IPR025121//IPR027417//IPR030394 - GTP binding domain // GTPase HflX // GTPase HflX, N-terminal // P-loop containing nucleoside triphosphate hydrolase // HflX-type guanine nucleotide-binding (G) domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGAGGAGGCGGAAGCCACGGGAGTAGTGCC
Internal bar code:CTGCGATGTCTACCACGGCGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:392
LEAP-Seq percent confirming:99.3232
LEAP-Seq n confirming:3669
LEAP-Seq n nonconfirming:25
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AGCGCTTGCTCAATTACCAT
Suggested primer 2:TTCACCTAGCCTCTGGCTGT