Insertion junction: LMJ.RY0402.084839_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.084839
Chromosome:chromosome 1
Location:3835015
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g024800 (1 of 2) PF00059//PF07714//PF14295 - Lectin C-type domain (Lectin_C) // Protein tyrosine kinase (Pkinase_Tyr) // PAN domain (PAN_4) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGACCGGAAGAGTCACCATGATGGCTGTG
Internal bar code:TAAGCACGCGACGGCAAGACGC

Confirmation - LEAP-Seq

LEAP-Seq distance:706
LEAP-Seq percent confirming:99.1647
LEAP-Seq n confirming:2137
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:CACATTGTCGGGCTTAAGGT
Suggested primer 2:AATCATCGTAGGCTTCGTGG