Insertion junction: LMJ.RY0402.084841_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.084841
Chromosome:chromosome 6
Location:2372493
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g267700 SPPA1-2,SPP1B,SPP2 Signal peptide peptidase; (1 of 1) PF00574//PF01343 - Clp protease (CLP_protease) // Peptidase family S49 (Peptidase_S49) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACTGTGCGCGCGAATGCGCAAGCAATGCG
Internal bar code:TATCGGGTCGTTTTCACAAGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:681
LEAP-Seq percent confirming:83.828
LEAP-Seq n confirming:7231
LEAP-Seq n nonconfirming:1395
LEAP-Seq n unique pos:51

Suggested primers for confirmation by PCR

Suggested primer 1:AGGAAAACAAAGCGCAAAGA
Suggested primer 2:TTCTCTGTCTGCAATCACCG