| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.084855 |
| Chromosome: | chromosome 4 |
| Location: | 2851606 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g224300 | CGL84 | (1 of 22) PTHR10994 - RETICULON; Conserved in the Green Lineage | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTGACGCTGCAACGATGTATGTCCATAA |
| Internal bar code: | TTATGGGATGGCAGAATGCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 645 |
| LEAP-Seq percent confirming: | 99.7101 |
| LEAP-Seq n confirming: | 344 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGACCGGTTAGGCTTTTG |
| Suggested primer 2: | GCGATTGACAGGATGGAGAT |