Insertion junction: LMJ.RY0402.084891_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.084891
Chromosome:chromosome 16
Location:6009954
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre16.g676450 CPLD39 Conserved in the Plant Lineage and Diatoms intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTGCGATAAGAGCTCCTGCGACTCGTAGG
Internal bar code:TGACCCACGCTTACCGGCCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:314
LEAP-Seq percent confirming:24.2962
LEAP-Seq n confirming:1096
LEAP-Seq n nonconfirming:3415
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:AAGTGCAATCGTTACCGACC
Suggested primer 2:GCCCTAAAAACCTCGGATTC