| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.084918 |
| Chromosome: | chromosome 6 |
| Location: | 3963011 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278192 | (1 of 1) PTHR15627:SF8 - DTW DOMAIN-CONTAINING PROTEIN 1 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCGGCACTGTTGCCACCAAGCGCTGAGC |
| Internal bar code: | AAGTGCGGTTACGCGCGGCCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 188 |
| LEAP-Seq percent confirming: | 96.7861 |
| LEAP-Seq n confirming: | 1837 |
| LEAP-Seq n nonconfirming: | 61 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGGAAGTGCTCCTCAATC |
| Suggested primer 2: | GCACTTTGGCTTTGGTTCTC |