Insertion junction: LMJ.RY0402.084938_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.084938
Chromosome:chromosome 17
Location:1753131
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre17.g709000 (1 of 1) PTHR11319//PTHR11319:SF35 - G PROTEIN-COUPLED RECEPTOR-RELATED // SUBFAMILY NOT NAMED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAATGTGCCTTGTGCGCACGCGCGAAACGC
Internal bar code:CCCTTATGACACTGGGCTCACA

Confirmation - LEAP-Seq

LEAP-Seq distance:288
LEAP-Seq percent confirming:86.5896
LEAP-Seq n confirming:2247
LEAP-Seq n nonconfirming:348
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:AGGGCGACAACTACACCATC
Suggested primer 2:GTGTAGTCGGCCATGAAGGT