| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.084944 |
| Chromosome: | chromosome 16 |
| Location: | 3809349 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g689550 | PTK8 | Putative tyrosine kinase; (1 of 10) PTHR23257//PTHR23257:SF520 - SERINE-THREONINE PROTEIN KINASE // IP11267P | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGTGGGCCGAGAGAACGCTGGTAGGAAGCA |
| Internal bar code: | GCGTGCTCGCCAGCCCGGAACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1134 |
| LEAP-Seq percent confirming: | 99.5465 |
| LEAP-Seq n confirming: | 4171 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATGCAGGCAAGTGAAGTC |
| Suggested primer 2: | TACACTTGTACGCTGAGGCG |