| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.084950 |
| Chromosome: | chromosome 15 |
| Location: | 244837 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g640750 | MAP3K17 | (1 of 1) PTHR24356:SF201 - SERINE/THREONINE-PROTEIN KINASE YPK1-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCTGTGGGCACGGGCGGCGCGGCTGCGC |
| Internal bar code: | TAGCCTTGCACGCCCAGGGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 393 |
| LEAP-Seq percent confirming: | 11.6423 |
| LEAP-Seq n confirming: | 621 |
| LEAP-Seq n nonconfirming: | 4713 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGTGATGGCCAGATCTTC |
| Suggested primer 2: | GCAAGCTAGCCGATGAAAAC |