| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.084957 |
| Chromosome: | chromosome 8 |
| Location: | 1952777 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g367600 | ASL1,CYSL,CYSM,OASTL1 | (1 of 3) 2.5.1.47 - Cysteine synthase / OAS sulfhydrylase; O-acetylserine (Thiol)-lyase/cysteine synthase 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAAATCCGGCTTAAGGTGTGCGATGGGA |
| Internal bar code: | GCCGTTCGGCGTACTCTCGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 680 |
| LEAP-Seq percent confirming: | 99.373 |
| LEAP-Seq n confirming: | 951 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGGTGTGGACTGTGGACT |
| Suggested primer 2: | GAGGCTGCAATGCACATAGA |