| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.084999 |
| Chromosome: | chromosome 13 |
| Location: | 4863530 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g605650 | ALDH10,ALDH10A1,ALD7 | (1 of 1) 1.2.1.8 - Betaine-aldehyde dehydrogenase / Betaine aldehyde oxidase; Aldehyde dehydrogenase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCCCACTCCACCGCCTTCTCAATGTCTG |
| Internal bar code: | GCGGCTCTGTGCGGTTTGCCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 384 |
| LEAP-Seq percent confirming: | 99.3737 |
| LEAP-Seq n confirming: | 476 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGAGAAGAGGTGGGCAGAG |
| Suggested primer 2: | GCGTGTGTGTGTGTGTGTGT |