| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.085001 |
| Chromosome: | chromosome 6 |
| Location: | 5288138 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g282800 | ICL1 | Isocitrate lyase; (1 of 1) 4.1.3.1 - Isocitrate lyase / Isocitritase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACAATCGTTAGTCGTAACAAAACACGGTC |
| Internal bar code: | TAAACCATAGCAGACGAAGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 726 |
| LEAP-Seq percent confirming: | 80.7752 |
| LEAP-Seq n confirming: | 2084 |
| LEAP-Seq n nonconfirming: | 496 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGATGTCCTGTCACCTCAGA |
| Suggested primer 2: | GCTACTTCGACCTGGTCAGC |