Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.085001 |
Chromosome: | chromosome_6 |
Location: | 5288138 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre06.g282800 | ICL1 | Isocitrate lyase | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTGGGAGGACTGTTGCCGGCAGTTGTTTGC |
Internal bar code: | GACCCAATTAGTGAATATGATT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 656 |
LEAP-Seq percent confirming: | 99.4413 |
LEAP-Seq n confirming: | 890 |
LEAP-Seq n nonconfirming: | 5 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGATGTCCTGTCACCTCAGA |
Suggested primer 2: | GCTACTTCGACCTGGTCAGC |