| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.085007 |
| Chromosome: | chromosome 1 |
| Location: | 6199790 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g044300 | (1 of 3) PTHR30570:SF0 - PHOSPHATE-BINDING PROTEIN PSTS | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAGGTAGTATGGTAGTGTGGACTGCAGG |
| Internal bar code: | CGCAAGCGATAAAAACGGGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 179 |
| LEAP-Seq percent confirming: | 68.6852 |
| LEAP-Seq n confirming: | 3312 |
| LEAP-Seq n nonconfirming: | 1510 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGTTGCTCAGCATGAAAA |
| Suggested primer 2: | AAATCATTTCGTGTCTCCCG |