Insertion junction: LMJ.RY0402.085012_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.085012
Chromosome:chromosome 6
Location:3686539
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g278140 (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCTTCAGAAACCCACCACCAGCGGCACG
Internal bar code:TCCAGGTTGCACCTCTTTGTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:971
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:107
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:ACCTGAAGGACCTGTTGTGC
Suggested primer 2:CTGCCTTCTCTGTCACACCA