| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.085046 |
| Chromosome: | chromosome 5 |
| Location: | 2547981 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g236000 | (1 of 2) PTHR23257//PTHR23257:SF446 - SERINE-THREONINE PROTEIN KINASE // LIM DOMAIN-CONTAINING SERINE/THREONINE-PROTEIN KINASE DDB_G0286997-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCGCCGATCTTGACTCGGTCCTGCTGCA |
| Internal bar code: | GCGTGTCAGCGTTCACGCTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 348 |
| LEAP-Seq percent confirming: | 48.9722 |
| LEAP-Seq n confirming: | 1358 |
| LEAP-Seq n nonconfirming: | 1415 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGCTCAGTTTTCCAGTGC |
| Suggested primer 2: | CATACTCGAAGGGCACAGGT |