Insertion junction: LMJ.RY0402.085093_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.085093
Chromosome:chromosome 4
Location:490354
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g217930 (1 of 1) PF06911 - Senescence-associated protein (Senescence) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGGGCTGGGCCCTTGAAGTAAAATGCATT
Internal bar code:GTACGACGGGAATGTTTCGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:522
LEAP-Seq percent confirming:52.381
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GACGTGCCGATTGTTAAGGT
Suggested primer 2:CGCAAGAATTGCAAACTCAA