| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.085149 |
| Chromosome: | chromosome 8 |
| Location: | 512286 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g359000 | (1 of 32) IPR006502 - Protein of unknown function PDDEXK-like | CDS|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGCGAGCAAGGATTGGCCGAAAAGTCCC |
| Internal bar code: | CATATCAAACCGATCTGTGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 524 |
| LEAP-Seq percent confirming: | 95.2239 |
| LEAP-Seq n confirming: | 319 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTAAATACATTGGCGGCG |
| Suggested primer 2: | ACTCTGGTAATGGTGGTGGC |