| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.085230 |
| Chromosome: | chromosome_8 |
| Location: | 862588 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g361200 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TACTTTCCGAACACACACAGCACACCACCA |
| Internal bar code: | TGGGTCAAGGAGGCCCGAAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 376 |
| LEAP-Seq percent confirming: | 90.0341 |
| LEAP-Seq n confirming: | 12666 |
| LEAP-Seq n nonconfirming: | 1402 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCCTCCTTTGTTGCTGTC |
| Suggested primer 2: | CAAGCCGAATAGCGAATAGC |