| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.085253 |
| Chromosome: | chromosome 17 |
| Location: | 2490116 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g716050 | (1 of 1) PTHR20953:SF2 - PROTEIN SEEDLING PLASTID DEVELOPMENT 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAAGAGGAGGGAGGCGAGAGGGTATCTG |
| Internal bar code: | TGAACACTTACCCTATGCCAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 364 |
| LEAP-Seq percent confirming: | 96.791 |
| LEAP-Seq n confirming: | 6485 |
| LEAP-Seq n nonconfirming: | 215 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGGAGGCAGAGGAAGATG |
| Suggested primer 2: | CCAGCCCAAACTAAACCAAA |