| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.085322 |
| Chromosome: | chromosome 3 |
| Location: | 8839496 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g206705 | (1 of 3) PF10250 - GDP-fucose protein O-fucosyltransferase (O-FucT) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTGTGTGCGTGCATGTGTGCATGTGTAC |
| Internal bar code: | AATCGGCGCCATCTTCTGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 497 |
| LEAP-Seq percent confirming: | 37.5149 |
| LEAP-Seq n confirming: | 628 |
| LEAP-Seq n nonconfirming: | 1046 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGATCTGCCTCCGAGTAG |
| Suggested primer 2: | TCCTGTTTCCCTTTCCACAC |