| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.085346 |
| Chromosome: | chromosome 3 |
| Location: | 6537728 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g195500 | TGL9 | (1 of 5) PF01734 - Patatin-like phospholipase (Patatin); Putative triacylglycerol lipase | 5'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCTTACACCTGTCTGTGACGCATACTCA |
| Internal bar code: | GCAGGATGAGGGATTTGTGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 533 |
| LEAP-Seq percent confirming: | 99.6248 |
| LEAP-Seq n confirming: | 531 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCATGCAGCTCCCACAGTA |
| Suggested primer 2: | GACCACCACTGAATTTGGCT |