| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.085347 |
| Chromosome: | chromosome 7 |
| Location: | 549601 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g316400 | (1 of 10) PTHR12270:SF6 - GLYCOSYLTRANSFERASE-LIKE PROTEIN LARGE | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGCACGGCCAGCGCTGGTCCCCAGTCAC |
| Internal bar code: | TAAAAAATTATTCTTGGTCATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 135 |
| LEAP-Seq percent confirming: | 99.455 |
| LEAP-Seq n confirming: | 365 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAACCACTCTGCAACCCTA |
| Suggested primer 2: | AAAATAAGGTGCATCACGCC |