| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.085360 |
| Chromosome: | chromosome 11 |
| Location: | 919348 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467659 | (1 of 1) K15128 - mediator of RNA polymerase II transcription subunit 6 (MED6) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGTTACGCAAGCAAGCGCTTGCCTAACA |
| Internal bar code: | ATGTAATGGAATATGCATATGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 808 |
| LEAP-Seq percent confirming: | 99.4293 |
| LEAP-Seq n confirming: | 1568 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGAATGCTGACTGGTGAA |
| Suggested primer 2: | GGAACAAGTTGGATCGCATT |