| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.085360 |
| Chromosome: | chromosome 11 |
| Location: | 919370 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467659 | (1 of 1) K15128 - mediator of RNA polymerase II transcription subunit 6 (MED6) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTTGCTTTCACCCCTTGCGCGTTCCAAA |
| Internal bar code: | AGCCGCACCCGGTGTCATGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 354 |
| LEAP-Seq percent confirming: | 98.8158 |
| LEAP-Seq n confirming: | 6425 |
| LEAP-Seq n nonconfirming: | 77 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGAATGCTGACTGGTGAA |
| Suggested primer 2: | GGAACAAGTTGGATCGCATT |