| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.085378 |
| Chromosome: | chromosome 14 |
| Location: | 986254 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g614100 | GTR26,DGL1 | Dolichyl-diphosphooligosaccharide-protein glycosyltransferase; (1 of 1) K12670 - oligosaccharyltransferase complex subunit beta (WBP1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGCAGGTTATGCTCCAAAACACCGCCGG |
| Internal bar code: | TTAAGACGCGTCGGTCATACCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 539 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 88 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACCTTCTCGCTGCGATTC |
| Suggested primer 2: | TCTACCAACTTACCCGTGGC |