| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.085380 |
| Chromosome: | chromosome 10 |
| Location: | 2726660 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g438300 | LTH4 | (1 of 15) 3.4.21.72 - IgA-specific serine endopeptidase / Immunoglobulin A1 protease; LAT2 homolog | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAACGTGAGTGCCATTCGCATGCTTTCAG |
| Internal bar code: | TCCATGCTTTCAGTTCATAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 399 |
| LEAP-Seq percent confirming: | 99.6139 |
| LEAP-Seq n confirming: | 774 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATCTAGTGCTGCTGGTCG |
| Suggested primer 2: | GTTACGGTGCCACAGGTCTT |