| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.085393 |
| Chromosome: | chromosome 12 |
| Location: | 4070668 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g517681 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGCCAACCTGTGGGAGGAAGGCCTGACG |
| Internal bar code: | ACGTGCTTTAAGATCGCTTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 944 |
| LEAP-Seq percent confirming: | 99.6617 |
| LEAP-Seq n confirming: | 1473 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTGGGAGTGACATGGGAT |
| Suggested primer 2: | AATGTACCCGCTACAGTGCC |