| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.085397 |
| Chromosome: | chromosome 9 |
| Location: | 5204294 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g399326 | MITC3, MCP3 | (1 of 2) K15110 - solute carrier family 25 (mitochondrial 2-oxodicarboxylate transporter), member 21 (SLC25A21, ODC); Mitochondrial substrate carrier protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGTCGGCAATGGTGGCAACTTACTGTTA |
| Internal bar code: | GTGGTATGGGTGGGGTGGAATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 539 |
| LEAP-Seq percent confirming: | 94.0414 |
| LEAP-Seq n confirming: | 4861 |
| LEAP-Seq n nonconfirming: | 308 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCCTGTTGAGTTTCGGAG |
| Suggested primer 2: | ACTGCACCAGGCTTACTGCT |