| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.085449 |
| Chromosome: | chromosome_13 |
| Location: | 1321278 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g571300 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGCCGAGTCGCTTGGCGCTCGCCTGGAGGA |
| Internal bar code: | TGGCCGAAGTAATTCCAGTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 674 |
| LEAP-Seq percent confirming: | 98.9333 |
| LEAP-Seq n confirming: | 3710 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAACTGAACCATGTATGCG |
| Suggested primer 2: | TGCAGCAAACCAACATGAAT |