| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.085487 |
| Chromosome: | chromosome 17 |
| Location: | 2724631 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g718150 | Glycosyl transferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATGGCGGCGGCGGCGCCCGTGCGCCCGGA |
| Internal bar code: | TCGTCCTCTGCGTGCGCCGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 529 |
| LEAP-Seq percent confirming: | 69.4379 |
| LEAP-Seq n confirming: | 593 |
| LEAP-Seq n nonconfirming: | 261 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGGTGTTCGTTACTGGACT |
| Suggested primer 2: | TCGATCCGAGTCTGTCACTG |