| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.085504 |
| Chromosome: | chromosome 17 |
| Location: | 5991672 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g740600 | MOT50 | Predicted protein; (1 of 1) 3.4.21.4 - Trypsin / Beta-trypsin | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACCGGCGGCCCCCAGGATACCGCCGCCGA |
| Internal bar code: | TCGCTCAGTTAACTTGCTCCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 275 |
| LEAP-Seq percent confirming: | 32.9465 |
| LEAP-Seq n confirming: | 1022 |
| LEAP-Seq n nonconfirming: | 2080 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTCTTGCGAATAGTAGGC |
| Suggested primer 2: | GCGTGCTCATGTGTGCTACT |