Insertion junction: LMJ.RY0402.085505_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.085505
Chromosome:chromosome 3
Location:2476382
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre03.g159550 (1 of 7) IPR011989//IPR016024 - Armadillo-like helical // Armadillo-type fold CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGGACCACGGACCAAACCCTCTTCCTCCA
Internal bar code:GTAGGATTTGTCGAACGGGTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:82
LEAP-Seq percent confirming:98.8525
LEAP-Seq n confirming:603
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:ATCATGCATGGCAAGTGTGT
Suggested primer 2:GTCAGAATCTCTCCGGTCCA