| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.085518 |
| Chromosome: | chromosome 13 |
| Location: | 2636420 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g581500 | OPR41 | OctotricoPeptide Repeat protein 41 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCATGGGCGAAACATGTTAGCAGCGCG |
| Internal bar code: | GACTGACGTGTTATGACGGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 82 |
| LEAP-Seq percent confirming: | 85.9813 |
| LEAP-Seq n confirming: | 92 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGCATAGTGTAGGCGCTC |
| Suggested primer 2: | AATCGATATGTCCCCCTTCC |