| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.085524 |
| Chromosome: | chromosome 16 |
| Location: | 1536276 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g653350 | (1 of 1) 3.5.1.108 - UDP-3-O-acyl-N-acetylglucosamine deacetylase / UDP-3-O-acyl-GlcNAc deacetylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCTGCTTGCGCGCCTCCGCGTCCCCTGC |
| Internal bar code: | GGGGGAGTACGGGAACGGGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 14 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTTCGTTGGGCACATATC |
| Suggested primer 2: | CCCCCTATCACCAGAATGTG |