| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.085539 |
| Chromosome: | chromosome 2 |
| Location: | 5638675 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g109450 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGGTAACACGGGGCGTTCTCGTATAAG |
| Internal bar code: | GGGTTCCGACCCGTGACTGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 761 |
| LEAP-Seq percent confirming: | 98.8364 |
| LEAP-Seq n confirming: | 1359 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCTGCAAGCAGACATGTGG |
| Suggested primer 2: | AGGGACAACGTGAAAATTCG |