| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.085564 |
| Chromosome: | chromosome 9 |
| Location: | 2117495 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393650 | (1 of 1) PF00069//PF01590 - Protein kinase domain (Pkinase) // GAF domain (GAF) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTAGTTTAGCTAATGTACCCCTAAATCAT |
| Internal bar code: | CCAGATCTGCGATGGCGTAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 931 |
| LEAP-Seq percent confirming: | 99.7602 |
| LEAP-Seq n confirming: | 1248 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATGCCAGCAGACTAGGTG |
| Suggested primer 2: | GTGTACTTCGGCACCTGGAT |