| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.085593 |
| Chromosome: | chromosome 16 |
| Location: | 1400442 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g652150 | FBP4 | (1 of 1) K01103 - 6-phosphofructo-2-kinase / fructose-2,6-biphosphatase 3 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTACTTTGCTCCATCGTTCTCGTGCCAT |
| Internal bar code: | TGTGGGCTAGCTCCTGCGATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 471 |
| LEAP-Seq percent confirming: | 97.1857 |
| LEAP-Seq n confirming: | 2659 |
| LEAP-Seq n nonconfirming: | 77 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATGTGGAATAGGAATGGG |
| Suggested primer 2: | AACCTAACGCGTGTGAATCC |