| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.085598 |
| Chromosome: | chromosome 4 |
| Location: | 3248606 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g226650 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTCCTACGGGCTGTGGGACGCTATGGAT |
| Internal bar code: | GTTGACGCTCGAGCAAATGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 106 |
| LEAP-Seq percent confirming: | 97.4619 |
| LEAP-Seq n confirming: | 192 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGCACTTCTCAGTGGTTT |
| Suggested primer 2: | GGTACTGCCGGTGTTTCTGT |