| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.085611 |
| Chromosome: | chromosome 12 |
| Location: | 5943476 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g534400 | (1 of 18) IPR011051 - RmlC-like cupin domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCCTCAATGGGTCCCGAGCCGCCGTTGT |
| Internal bar code: | CCTCTCGAGCGGCCTCAAGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 930 |
| LEAP-Seq percent confirming: | 99.605 |
| LEAP-Seq n confirming: | 1513 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTACAGGGCAGGGTTAGG |
| Suggested primer 2: | AGGGATGGATGTCACATGGT |