| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.085640 |
| Chromosome: | chromosome 2 |
| Location: | 5884693 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g111426 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAACCATAGTAGCCAGAGTGGTACCCGCG |
| Internal bar code: | GCTCTCTACGCGTTGGTTACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 289 |
| LEAP-Seq percent confirming: | 97.4464 |
| LEAP-Seq n confirming: | 954 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACTCACGCGGGTACCACT |
| Suggested primer 2: | AACTGTCATGCAGAGCGATG |