| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.085644 |
| Chromosome: | chromosome 2 |
| Location: | 6355648 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g115350 | (1 of 2) 2.7.7.59 - [Protein-PII] uridylyltransferase / Uridylyl removing enzyme | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCACCCCCCCCAAACACTGGTCGCGTGC |
| Internal bar code: | CTCTTAGAGGGTTGCGACGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 551 |
| LEAP-Seq percent confirming: | 62.6648 |
| LEAP-Seq n confirming: | 20054 |
| LEAP-Seq n nonconfirming: | 11948 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTAGCGAAGTAAAGCGGAC |
| Suggested primer 2: | CGTAGGTCTTCATTTGGGGA |