| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.085645 |
| Chromosome: | chromosome 6 |
| Location: | 7795682 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g302450 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTAACTAGCGTACGGATGGCGGGGAGCTA |
| Internal bar code: | ACGCACGGGCTATAGGTGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 352 |
| LEAP-Seq percent confirming: | 97.8622 |
| LEAP-Seq n confirming: | 412 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGATGAGTGCGAGGAGGT |
| Suggested primer 2: | TTATTGCTGATTTCTGGGGC |