Insertion junction: LMJ.RY0402.085650_4

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.085650
Chromosome:chromosome 12
Location:2463211
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g506851 BBS3A (1 of 2) K07951 - ADP-ribosylation factor-like protein 6 (ARL6, BBS3); Bardet-Biedl syndrome-3 associated protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAGCCCGAGAACGCCGCCGGGTGAGGGGC
Internal bar code:GGGGCACCACAAACAACCAATA

Confirmation - LEAP-Seq

LEAP-Seq distance:701
LEAP-Seq percent confirming:82.7858
LEAP-Seq n confirming:9450
LEAP-Seq n nonconfirming:1965
LEAP-Seq n unique pos:47

Suggested primers for confirmation by PCR

Suggested primer 1:CGGTCCGAACAAACATAACC
Suggested primer 2:CCGGGAAAGTTTGTCACAGT