| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.085652 |
| Chromosome: | chromosome 13 |
| Location: | 2319734 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g579100 | APC1 | (1 of 1) K03348 - anaphase-promoting complex subunit 1 (APC1); Anaphase promoting complex subunit 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCGACACTACGAACTTGTCCTATACACAC |
| Internal bar code: | CGGCCAATGCCGGGCGTCGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 638 |
| LEAP-Seq percent confirming: | 99.61 |
| LEAP-Seq n confirming: | 10727 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGGTTTAGTTTGGGCTGG |
| Suggested primer 2: | GTCTGCGTGTCCCCTATCTC |